Open AccessB‐cell linker protein prevents aneuploidy by inhibiting cytokinesis
Author(s) -
Kamino Hiroki,
Futamura Manabu,
Nakamura Yasuyuki,
Kitamura Noriaki,
Kabu Koki,
Arakawa Hirofumi
Publication year - 2008
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/j.1349-7006.2008.00990.x
Subject(s) - cytokinesis , biology , microbiology and biotechnology , midbody , aneuploidy , cancer research , ectopic expression , cell , cell culture , cell division , genetics , gene , chromosome
Aneuploidy is a hallmark of human cancers. Although the maintenance of genomic integrity by p53 is important in preventing aneuploidy, its mechanism remains to be elucidated. Here we report evidence that B‐cell linker protein (BLNK) mediates the inhibition of cytokinesis, which generates tetraploidy but prevents aneuploidy. We identified BLNK as a transcriptional target of p53. Surprisingly, ectopic expression of exogenous BLNK inhibited cytokinesis, resulting in the formation of tetraploid cells. Indeed, BLNK was involved in the generation of spontaneously arising binucleate tetraploid cells. Interestingly, cytokinesis after DNA damage was inhibited in p21 −/– and p53 +/+ cells, but not in p53 −/– cells. BLNK knockdown in p53 +/+ and p21 −/– cells enhanced cytokinesis after DNA damage, leading to the generation of aneuploid cells. In addition, a BLNK‐downregulated human pre‐B leukemia cell line showed increased cytokinesis and aneuploidy after DNA damage compared with two other pre‐B leukemia cell lines expressing higher levels of BLNK. These results suggest that BLNK acts as a mediator of p53 in the inhibition of cytokinesis, which prevents aneuploidy. We propose that the inhibition of cytokinesis is crucial for the maintenance of genomic integrity. ( Cancer Sci 2008; 99: 2444–2454)