Open AccessREG I enhances chemo‐ and radiosensitivity in squamous cell esophageal cancer cells
Author(s) -
Hayashi Kaori,
Motoyama Satoru,
Koyota Souichi,
Koizumi Yukio,
Wang Jingshu,
Takasawa Shin,
ItayaHironaka Asako,
SakuramotoTsuchida Sumiyo,
Maruyama Kiyotomi,
Saito Hajime,
Minamiya Yoshihiro,
Ogawa Junichi,
Sugiyama Toshihiro
Publication year - 2008
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/j.1349-7006.2008.00980.x
Subject(s) - radiosensitivity , transfection , cell cycle , cancer research , flow cytometry , squamous carcinoma , esophageal cancer , cell , cancer , cancer cell , biology , cell culture , medicine , carcinoma , pathology , microbiology and biotechnology , radiation therapy , genetics
Identification of reliable markers of chemo‐ and radiosensitivity and the key molecules that enhance the susceptibility of squamous esophageal cancer cells to anticancer treatments would be highly desirable. To test whether regenerating gene ( REG ) I expression enhances chemo‐ and radiosensitivity in esophageal squamous cell carcinoma cells, we used MTT (3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyltetrazolium bromide) assays to compare the chemo‐ and radiosensitivities of untransfected TE‐5 and TE‐9 cells with those of cells stably transfected with REG Iα and Iβ. We then used flow cytometry to determine whether REG I expression alters cell cycle progression. No REG I mRNA or protein were detected in untransfected TE‐5 and TE‐9 cells. Transfection with REG Iα and Iβ led to strong expression of both REG I mRNA and protein in TE‐5 and TE‐9 cells, which in turn led to significant increases in both chemo‐ and radiosensitivity. Cell cycle progression was unaffected by REG I expression. REG I thus appears to enhance the chemo‐ and radiosensitivity of squamous esophageal cancer cells, which suggests that it may be a useful target for improved and more individualized treatments for patients with esophageal squamous cell carcinoma. ( Cancer Sci 2008; 99: 2491–2495)