Open AccessDifferential expression of the human α‐enolase gene in oral epithelium and squamous cell carcinoma
Author(s) -
Ito Satoshi,
Honma Takayoshi,
Ishida Kosei,
Wada Naoyuki,
Sasaoka Shunsuke,
Hosoda Masaru,
Nohno Tsutomu
Publication year - 2007
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/j.1349-7006.2007.00411.x
Subject(s) - immunostaining , epithelium , biology , in situ hybridization , cytoplasm , carcinogenesis , pathology , microbiology and biotechnology , gene expression , immunohistochemistry , epidermoid carcinoma , carcinoma , gene , immunology , medicine , biochemistry , genetics
α‐Enolase and c‐ myc promoter binding protein 1 are encoded by a single gene, ENO1 , and are synthesized from the same transcript through alternative use of translational start sites. We have investigated the localization of ENO1 gene transcripts detected as proteins with an immunohistochemical method and also as mRNA with an in situ hybridization method on tissue sections of oral epithelium and oral squamous cell carcinoma, and demonstrated the differential distribution of the gene transcripts in normal oral epithelium and oral squamous cell carcinoma in humans. Expression of the ENO1 transcript was detectable in the region from the basal cell layers to the lower granular cell layers. Three patterns of ENO1 localization were observed with immunostaining in the epithelia: cytoplasm, nuclei, and both nuclei and cytoplasm. These patterns were observed randomly within the same specimen. In contrast to normal oral epithelium, ENO1 protein was not detectable in the nuclei of carcinoma cells. Our results indicate that differential subcellular localization of ENO1 products may be closely related to carcinogenesis of the oral epithelium. ( Cancer Sci 2007; 98: 499–505)