Open AccessInfrequent existence of simian virus 40 large T antigen DNA in malignant mesothelioma in Japan
Author(s) -
Aoe Keisuke,
Hiraki Akio,
Murakami Tomoyuki,
Toyooka Shinichi,
Shivapurkar Narayan,
Gazdar Adi F.,
Sueoka Naoko,
Taguchi Koji,
Kamei Toshiaki,
Takeyama Hiroyasu,
Sugi Kazuro,
Kishimoto Takumi
Publication year - 2006
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/j.1349-7006.2006.00171.x
Subject(s) - mesothelioma , sv40 large t antigen , simian , virus , polymerase chain reaction , antigen , pathology , virology , neoplasm , biology , medicine , immunology , cell culture , gene , transfection , genetics , biochemistry
Malignant mesothelioma is the most common primary pleural neoplasm. Association of simian virus 40 (SV40) with malignant mesothelioma has been reported, suggesting that SV40 plays an important role in the origin of a subset of these tumors. However, significant geographic variation is present as to how often this association occurs. As no study concerning SV40 in malignant mesothelioma has been reported from Japan, we examined the frequency of SV40 infection in Japanese malignant mesothelioma cases. In pleural malignant mesothelioma tissue from 35 patients in Japan, we sought the presence of SV40 large T antigen DNA using real‐time polymerase chain reaction (PCR), as well as expression of the viral protein using immunohistological methods. Real‐time PCR demonstrated that two of 35 mesotheliomas contained DNA sequences encoding portions of SV40 large T antigen. None of the 35 malignant mesothelioma specimens showed immunoreactivity for SV40 large T antigen. SV40 infection does not appear to have a major role in the development of malignant mesothelioma in Japan. ( Cancer Sci 2006; 97: 292–295)