Open AccessIncreased expression of integrin α3β1 in highly brain metastatic subclone of a human non‐small cell lung cancer cell line
Author(s) -
Yoshimasu Tatsuya,
Sakurai Teruhisa,
Oura Shoji,
Hirai Issei,
Tanino Hirokazu,
Kokawa Yozo,
Naito Yasuaki,
Okamura Yoshitaka,
Ota Ichiro,
Tani Naoyuki,
Matsuura Nariaki
Publication year - 2004
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/j.1349-7006.2004.tb03195.x
Subject(s) - integrin , bone metastasis , brain metastasis , laminin , metastasis , pathology , cancer research , cell culture , cell migration , extracellular matrix , lung cancer , biology , cell , chemistry , medicine , cancer , microbiology and biotechnology , genetics
To clarify the roles of integrin and extracellular matrix (ECM) in the process of non‐small cell lung cancer (NSCLC) brain metastasis, we established an in vivo model of brain metastasis of human NSCLC cell line EBC‐1/original in athymic mice, and established highly brain metastatic subclone EBC‐1/brain and highly bone metastatic subclone EBC‐1/bone. Integrin expression of these subclones was evaluated by flow cytometry. In vitro cell attachment, migration and proliferation assays with ECMs were performed using these subclones. Expression of integrin α3 subunit was higher in EBC‐1/brain than in both EBC‐1/original and EBC‐1/bone. In vitro cell attachment, migration, and proliferation assays revealed that EBC‐1/brain had higher affinity and higher reactivity to laminin than EBC‐1/original and EBC‐1/bone. Blocking of integrin α3β1 significantly (P<0.05) decreased brain metastasis by EBC‐1/brain. Interaction of integrin α3β1 and laminin plays important roles in the process of brain metastasis of non‐small cell lung cancer.