Open AccessIncreased expansion of Vα24 + T cells derived from G‐CSF‐mobilized peripheral blood stem cells as compared to peripheral blood mononuclear cells following α‐galactosylceramide stimulation
Author(s) -
AsadaMikami Rumiko,
Heike Yuji,
Harada Yukie,
Kanai Sachiyo,
Ikarashi Yoshinori,
Kato Kazunori,
Shirakawa Kazuo,
Takaue Yoichi,
Abe Tatsuo,
Wakasugi Hiro
Publication year - 2003
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/j.1349-7006.2003.tb01451.x
Subject(s) - peripheral blood mononuclear cell , peripheral blood , stimulation , peripheral , stem cell , peripheral blood stem cells , medicine , pathology , immunology , chemistry , endocrinology , biology , microbiology and biotechnology , in vitro , biochemistry , haematopoiesis
In the present study, unpurified peripheral blood mononuclear cells (PBMCs) from various sources, including steady‐state blood (normal donors) and granulocyte colony‐stimulating factor (G‐CSF)‐mobilized blood (cancer patients and normal donors) (G‐PBSC), were cultured in RPMI‐1640 in the presence of IL‐2 and α‐galactosylceramide (α‐GalCer) to expand Vα24 + T cells, and their expansion kinetics were compared. G‐CSF‐mobilized cells showed markedly higher expansion potential (350‐fold expansion of Vα24 + T cells, regardless of whether the cells were from cancer patients or normal donors) than steady‐state cells (15‐fold expansion, compared to the initial inoculums) ( n =5, P <0.01). We also confirmed that the CD14 ‐ fraction of G‐PBSCs contained a large number of precursors of Vα24 + T cells, compared to PBSCs, as well as a large number of CD14+ cells, which assist Vα24 + T cell proliferation. Our simple and practical procedure, which eliminates complicated cell manipulation (including cell purification), produces efficient expansion of Vα24 + T cells when G‐CSF‐mobilized blood cells are cultured with α‐GalCer. (Cancer Sci 2003; 94: 383–388)