Open AccessScreening of genes specifically activated in the pancreatic juice ductal cells from the patients with pancreatic ductal carcinoma
Author(s) -
Yoshida Koji,
Ueno Shuichi,
Iwao Toshiyasu,
Yamasaki Souichirou,
Tsuchida Akira,
Ohmine Ken,
Ohki Ruri,
Choi Young Lim,
Koinuma Koji,
Wada Tomoaki,
Ota Jun,
Yamashita Yoshihiro,
Chayama Kazuaki,
Sato Kazuhiro,
Mano Hiroyuki
Publication year - 2003
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/j.1349-7006.2003.tb01431.x
Subject(s) - ductal cells , microarray , pancreatic juice , biology , ductal carcinoma , dna microarray , gene , pancreatic cancer , population , transcriptome , microarray analysis techniques , pancreas , cancer research , gene expression , pathology , medicine , genetics , cancer , endocrinology , environmental health , breast cancer
Pancreatic ductal carcinoma (PDC) is one of the most intractable human malignancies. Surgical resection of PDC at curable stages is hampered by a lack of sensitive and reliable detection methods. Given that DNA microarray analysis allows the expression of thousands of genes to be monitored simultaneously, it offers a potentially suitable approach to the identification of molecular markers for the clinical diagnosis of PDC. However, a simple comparison between the transcriptomes of normal and cancerous pancreatic tissue is likely to yield misleading pseudopositive data that reflect mainly the different cellular compositions of the specimens. Indeed, a microarray comparison of normal and cancerous tissue identified the INSULIN gene as one of the genes whose expression was most specific to normal tissue. To eliminate such a “population‐shift” effect, the pancreatic ductal epithelial cells were purified by MUC1‐based affinity chromatography from pancreatic juice isolated from both healthy individuals and PDC patients. Analysis of these background‐matched samples with DNA microarrays representing 3456 human genes resulted in the identification of candidate genes for PDC‐specific markers, including those for AC133 and carcinoembryonic antigen‐related cell adhesion molecule 7 ( CEACAM7 ). Specific expression of these genes in the ductal cells of the patients with PDC was confirmed by quantitative real‐time polymerase chain reaction analysis. Microarray analysis with purified pancreatic ductal cells has thus provided a basis for the development of a sensitive method for the detection of PDC that relies on pancreatic juice, which is routinely obtained in the clinical setting. (Cancer Sci 2003; 94: 263–270)