Open AccessNot Infrequent K‐ ras Mutations in Depressed‐type Early Colorectal Carcinomas Larger than 10 mm
Author(s) -
Kazama Shinsuke,
Ajioka Yoichi,
Watanabe Hidenobu,
Watanabe Toshiaki,
Nagawa Hirokazu
Publication year - 2002
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.2002.tb01256.x
Subject(s) - microdissection , biology , mutation , polymerase chain reaction , restriction fragment length polymorphism , point mutation , microbiology and biotechnology , genetics , gene
The aim of this study was to elucidate whether K‐ ras (codons 12 and 13) mutations occur in depressed‐type early colorectal carcinomas (DECas) larger than 10 mm in size. Thirty‐four cases of DECas including 27 larger than 10 mm were examined for K‐ ras mutations by means of microdis‐section, PCR‐RFLP (polymerase chain reaction‐restriction fragment length polymorphism), and direct sequencing. Although K‐ ras mutation was infrequent (1/7, 14%) in small (less than 10 mm) DECas, 16/27 (59%) and 17/27 (63%) of DECas larger than 10 mm revealed codon 12, or either codon 12 or 13 mutations, respectively. None of the evaluated pathological factors except size showed a correlation with K‐ ras mutation. These data indicate that although K‐ ras mutation could not be involved in the early stage of the progression of DECas, it might play a role at a later stage when the tumor size is over 10 mm.