Open AccessA Radicicol Derivative, KF58333, Inhibits Expression of Hypoxia‐inducible Factor‐1α and Vascular Endothelial Growth Factor, Angiogenesis and Growth of Human Breast Cancer Xenografts
Author(s) -
Kurebayashi Junichi,
Otsuki Takemi,
Kurosumi Masafumi,
Soga Shiro,
Akinaga Shiro,
Sonoo Hiroshi
Publication year - 2001
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.2001.tb02159.x
Subject(s) - angiogenesis , vascular endothelial growth factor , hif1a , biology , cancer research , growth factor , endocrinology , receptor , biochemistry , vegf receptors
A novel oxime derivative of radicicol, KF58333, binds to the heat shock protein 90 (Hsp90) and destabilizes its associated signaling molecules. These effects play a critical role in the growth inhibition of tumor cells. To further investigate the effects of this agent, it was administered to two human breast cancer cell lines, KPL‐1 and KPL‐4, both in vitro and in vivo. KF58333 dose‐dependently inhibited the growth and vascular endothelial growth factor (VEGF) secretion, concomitantly with a decrease in VEGF mRNA expression, in each cell line. This agent also suppressed the increase of VEGF secretion and expression induced by hypoxia (1% O 2 ). Intravenous injections of this agent into nude mice bearing either KPL‐1 or KPL‐4 xenografts significantly inhibited the tumor growth associated with a decrease in the Ki67 labeling index and microvascular area and an increase in apoptosis and the necrotic area. These findings indicate that the antitumor activity of this radicicol derivative may be partly mediated by decreasing VEGF secretion from tumor cells and inhibiting tumor angiogenesis. To explore the action mechanisms of the anti‐angiogenic effect, the expression level of hypoxia‐inducible factor (HIF)‐lα was investigated. KF58333 provided a significant decrease in the HTF‐lα protein expression under both normoxic and hypoxic conditions. In contrast, the mRNA expression of HIF‐lα was not decreased by this agent. It is suggested that the post‐transcriptional down‐regulation of HIF‐lα expression by this agent may result in a decrease of VEGF expression and tumor angiogenesis.