Open AccessAberrations of the p14 ARF and p16 INK4a Genes in Renal Cell Carcinomas
Author(s) -
Kawada Yoichi,
Nakamura Mitsutoshi,
Ishida Eiwa,
Shimada Keiji,
Oosterwijk Egbert,
Uemura Hirotsugu,
Hirao Yoshihiko,
Chul Kim Sung,
Konishi Noboru
Publication year - 2001
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.2001.tb02152.x
Subject(s) - cancer research , renal cell carcinoma , biology , microbiology and biotechnology , chemistry , medicine , pathology
The INK4alARF locus on chromosome 9p21, which encodes two distinct genes, p14 ARF and p16 INK4a , is frequently altered in human neoplasms. To investigate the potential roles of p14 ARF and p16 IfiK4a genes in human renal cell carcinomas (RCCs), we analyzed 6 human RCC cell lines and 91 primary RCCs for homozygous deletion, promoter hypermethylation and expression of the p14 ARF and p16 INK4a gene products using differential PCR, methylation‐specific PCR, and immunohistochemis‐try, respectively. Five cell lines showed homozygous co‐deletion of both genes and one demonstrated promoter hypermethylation of the p16 INK4a gene only. Eight of 91 RCCs showed aberrations of p14 ARF or p16 INK4a status and six of these featured gross extension into the renal vein. The results suggest that p14 ARF and p16 INK4a aberrations may play roles in the relatively late stage of renal tumorigenesis associated with tumor progression.