Open AccessFive Quantitative Trait Loci Affecting 4‐Nitroquinoline 1‐Oxide‐induced Tongue Cancer in the Rat
Author(s) -
Tanuma Junichi,
Fujii Kei,
Hirano Masato,
Matsuuchi Hiroaki,
Shisa Hayase,
Hiai Hiroshi,
Kitano Motoo
Publication year - 2001
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.2001.tb01138.x
Subject(s) - quantitative trait locus , 4 nitroquinoline 1 oxide , biology , genetics , allele , tongue , backcrossing , phenotype , carcinogenesis , cancer , gene , pathology , medicine
In our previous study, Dark‐Agouti (DA) rats were found to be highly susceptible to 4‐nitroquino‐line 1‐oxide (4NQO)‐induced tongue carcinoma (TC), whereas Wistar/Furth (WF) rats were barely susceptible. Interval mapping analysis of reciprocal backcross rats showed two quantitative trait loci (QTL) on rat chromosomes (RNO) 1 and 19. In the present study, a composite interval mapping analysis was applied to 4NQO‐induced TC in 130 (DAxWF) F2 rats, demonstrating five independent QTL, Tongue squamous cell carcinoma 1‐5 (Tscc1‐5 ), responsible for phenotypic differences in the size and number of TCs in the two strains. Two of these QTL were mapped on RNO1, and the others were mapped on RNO4, 14, and 19. The DA allele at these loci consistently yielded semidominant susceptibility to TC. Out of the five loci detected in this F2 generation, Tscc1 and 2 were identical to Stcl and Rtc1 described in our previous study, but the other three were novel. We propose a new nomenclature consistent with their function. Genome‐wide screening of the F2 progeny also suggested the presence of three additional QTL on RNO5, 6, and 10. The possible roles of these loci in tongue carcinogenesis are discussed.