Open AccessReduced Expression of Laminin α3 and α5 Chains in Non‐small Cell Lung Cancers
Author(s) -
Akashi Takumi,
Ito Eisaku,
Eishi Yoshinobu,
Koike Morio,
Nakamura Kyoichi,
Burgeson Robert E.
Publication year - 2001
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.2001.tb01094.x
Subject(s) - laminin , adenocarcinoma , pathology , basement membrane , biology , stromal cell , alpha (finance) , cancer research , lung cancer , cancer , microbiology and biotechnology , extracellular matrix , medicine , construct validity , nursing , patient satisfaction , genetics
The basement membrane is considered to act as a barrier which hinders cancer cells from invading the surrounding stroma. In order to assess changes in essential components during neoplasia in the lung, we immunohistochemically studied distribution patterns of laminins α3 and α5 in 40 adeno‐carcinomas and 8 squamous cell carcinomas. The α5 chain was generally preserved at the periphery, frequently disrupted in foci with alveolar collapse and absent in foci of fibroblastic proliferation within adenocarcinomas. Fragmentation and absence of laminin α3 chain were more prominent than for α5 chain. Laminin α3 chain was partially fragmented or absent in peripheral areas of adenocarcinomas, being significantly different from α5 chain. Non‐small cell lung cancers with reduced α5 chain showed a tendency for greater lymph node metastasis. In cultured normal air way epithelial cells, both laminin α3 and α5 chains were found to be expressed by northern analysis. Eleven of the twelve cultured lung cancer cell lines did not express a3 chain and expression of α5 chain was reduced in three. Quantitative RT‐PCR analysis also demonstrated expression of laminin α3 chain in adenocarcinoma tissues to be significantly lower than in normal lung tissues. These results suggest that expression of laminin a chains is often reduced in lung cancer cells and this might contribute to basement membrane fragmentation and subsequent proliferation of stromal elements, as well as play some role in the process of cancer cell invasion.