Open AccessFusion of a Sequence from HEI10 (14q11) to the HMGIC Gene at 12ql5 in a Uterine Leiomyoma
Author(s) -
Mine Nobuya,
Kurose Keisuke,
Konishi Hideki,
Araki Tsutomu,
Nagai Hisaki,
Emi Mitsuru
Publication year - 2001
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.2001.tb01075.x
Subject(s) - uterine leiomyoma , biology , leiomyoma , complementary dna , fusion gene , gene , exon , genetics , uterus , pathology , medicine
Uterine leiomyoma, a benign smooth‐muscle tumor of the myometrium, is the most commonly encountered neoplasm in women of reproductive age. Band q15 of chromosome 12 is often rearranged in benign mesenchymal tumors such as uterine leiomyomas, and the HMGIC gene, encoding a protein of the high‐mobility‐group (HMG), is present in that region. Using 3′ rapid amplification of cDNA ends (3RACE) experiments, we isolated an ectopic sequence that was fused to HMGIC in a uterine leiomyoma. Cloning of the fusion cDNA identified a gene termed “homo sapiens enhancer of invasion 10” ( HEI10 ) as the fusion partner. Radiation hybrid mapping revealed that the normal location of HEI10 is at 14qll. In the fusion transcript the first two exons of the HMGIC gene, which encode DNA‐binding domains, were fused to the 3’portion of the HEI10 gene. This rearrangement implicates HMGIC in the tumorigenesis of uterine leiomyoma, and suggests that its fusion HMGIC product may play a role in mesenchymal differentiation.