Open AccessAbsence of Germline CHK2 Mutations in Familial Gastric Cancer
Author(s) -
Kimura Kenji,
Shinmura Kazuya,
Yoshimura Kimio,
Shimizu Kimihiro,
Katai Hitoshi,
Beppu Yasuo,
Moriya Hideshige,
Yokota Jun
Publication year - 2000
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.2000.tb01028.x
Subject(s) - germline , germline mutation , genetics , cancer , biology , mutation , gene , cancer research , coding region
Recently, the CHK2 gene was identified as being a candidate gene responsible for Li‐Fraumeni syndrome (LFS). Gastric cancer is often clustered in families with LFS, so it is possible that germline CHK2 mutation is also present in familial gastric cancer (FGC). We therefore defined the genomic structure of the CHK2 gene, designed intronic primers, and searched for germline CHK2 mutations in 25 FGC cases by polymerase chain reaction‐single strand conformational polymorphism analysis of the entire coding region. In all of the 25 cases, at least two siblings had histories of gastric cancer. There were no FGC cases that showed germline CHK2 mutations. Thus, it was indicated that germline CHK2 mutations do not contribute to the familial clustering of gastric cancer.