Isolation of Two Novel Genes, Down‐regulated in Gastric Cancer | Zendy

Yoshikawa Yoshie | Zendy; Mukai Hiroyuki | Zendy; Hino Fumitsugu | Zendy; Asada Kiyozo | Zendy; Kato Ikunoshin | Zendy

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Isolation of Two Novel Genes, Down‐regulated in Gastric Cancer

Author(s) -

Yoshikawa Yoshie,

Mukai Hiroyuki,

Hino Fumitsugu,

Asada Kiyozo,

Kato Ikunoshin

Publication year - 2000

Publication title -

japanese journal of cancer research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.035

H-Index - 141

eISSN - 1349-7006

pISSN - 0910-5050

DOI - 10.1111/j.1349-7006.2000.tb00967.x

Subject(s) - cancer , gene , biology , stomach , stomach cancer , gene expression , blot , cancer cell , microbiology and biotechnology , cancer research , cell culture , gastric mucosa , northern blot , pathology , genetics , medicine , biochemistry

Using a differential display technique, we identified two genes that are down‐regulated in human gastric cancer tissue as compared to normal gastric mucosa. The down‐regulated expression of these genes in gastric cancer tissue was confirmed by northern blotting analysis and RT‐PCR. One, CA11, was a novel gene expressed predominantly in the stomach and was depleted in all of the gastric cancer cell lines examined. The other gene, GC36, was homologous to the digestive tract‐specific calpain gene, nCL‐4. The expression of both GC36 and nCL‐4 was suppressed or depleted in gastric cancer cell lines of differentiated and poorly differentiated types. This is the first report of genes, the expression of which is down‐regulated with considerable frequency in gastric cancer.

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