Open AccessL‐myc Restriction Fragment Length Polymorphism in Japanese Patients with Esophageal Cancer
Author(s) -
Shibuta Kenji,
Inoue Hiroshi,
Sato Koichi,
Matsuyama Ayumi,
Ueo Hiroaki,
Tanaka Youichi,
Mafune Kenichi,
Barnard Graham F.,
Mori Masaki
Publication year - 2000
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.2000.tb00932.x
Subject(s) - genotype , genotyping , esophageal cancer , allele , restriction fragment length polymorphism , biology , gastroenterology , polymerase chain reaction , medicine , allele frequency , genetics , cancer , gene
L‐myc polymorphism is a representative genetic trait related to an individual's susceptibility to several cancers. However, there have been no reports concerning the association between esophageal cancer and L‐myc polymorphism. To analyze the distribution of polymorphism in Japanese patients with esophageal cancer, a molecular genotyping method using a polymerase chain reaction‐based restriction fragment length polymorphism (PCR‐RFLP) was used. Based on an analysis of 65 Japanese patients with esophageal cancer and 107 healthy control subjects, a significant difference was observed in either the distribution of genotypes ( P =0.012) or of allele frequencies between the two groups ( P 0.004). The relative risk of esophageal cancer for genotypes including the shorter allele was 2.9 compared to the longer allele homozygote. Furthermore, the patients with S‐allele had a tendency for poor prognosis among those with three genotypes. A significant difference between the distribution of genotypes and the incidence of lymph node metastasis was found based on the clinicopathological features of the cancers. These results suggest that L‐myc polymorphism may be implicated as a genetic trait affecting an individual's susceptibility to esophageal cancer, at least among Japanese patients.