Open AccessThe Characteristics of Epstein‐Barr Virus (EBV)‐positive Diffuse Large B‐Cell Lymphoma: Comparison between EBV + and EBV ‐ Cases in Japanese Population
Author(s) -
Kuze Tetuo,
Nakamura Naoya,
Hashimoto Yuko,
Sasaki Yoshikazu,
Abe Masafumi
Publication year - 2000
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.2000.tb00909.x
Subject(s) - epstein–barr virus , lymphoma , virus , diffuse large b cell lymphoma , gammaherpesvirinae , herpesviridae , population , virology , medicine , viral disease , immunology , environmental health
We have investigated 114 cases with diffuse large B‐cell lymphoma (DLBCL) to clarify the characteristics of DLBCL with Epstein‐Barr virus (EBV) infection. Thirteen cases (11.4%) showed EBVencoded RNA 1 (EBER1) signals by RNA in situ hybridization. EBV‐encoded latent membrane protein 1 (LMP1) and EBV‐encoded nuclear antigen 2 (EBNA2) were expressed in 11 and 4 cases, respectively. Expression of CD30, Bcl‐6 and immunoglobulin (Ig) was found in 92%, 31% and 23% with EBV + DLBCL, and in 15%, 79% and 82% with EBV ‐ DLBCL, respectively. The sequence of rearranged Ig heavy chain (IgH) variable (V) region gene was analyzed in 5 cases with EBV + DLBCL and 61 cases with EBV ‐ DLBCL. Somatic mutation was found in all cases except one with EBV ‐ DLBCL. Average mutation frequency was 9.6% in EBV + DLBCL vs. 11.5% in EBV ‐ DLBCL. The rates of replacement mutation vs. silent mutation (R/S values) in complementarity determining region II and framework region III were 2.7 and 1.5 in EBV + DLBCL, 2.6 and 1.4 in EBV ‐ DLBCL. Crippling mutation generating a stop codon was found in 2 of 5 cases (40%) with EBV + DLBCL, but none of 61 cases (0%) with EBV ‐ DLBCL. These findings suggest that EBV + DLBCL and EBV ‐ DLBCL were both derived from germinal center (GC) or post‐GCB cells, and EBV + DLBCL frequently have a non‐functional IgH gene owing to crippling mutation.