Open AccessIntratumor Heterogeneity of Centromere Numerical Abnormality in Multiple Primary Gastric Cancers: Application of Fluorescence in situ Hybridization with Intermittent Microwave Irradiation on Paraffin‐embedded Tissue
Author(s) -
Kobayashi Keiko,
Kitayama Yasuhiko,
Igarashi Hisaki,
Yoshino Goro,
Kobayashi Toshihiko,
Kazui Teruhisa,
Sugimura Haruhiko
Publication year - 2000
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.2000.tb00896.x
Subject(s) - centromere , fluorescence in situ hybridization , in situ , pathology , biology , in situ hybridization , abnormality , chromosome , chromosome instability , medicine , chemistry , genetics , gene , gene expression , organic chemistry , psychiatry
Our recent success in retrieving distinct fluorescence signals in response to centromere specific probing of paraffin‐embedded tissues after intermittent microwave (MW) treatment provided the opportunity to analyze chromosome numbers or centromere abnormality in situ in human tumors in various clinicopathological settings. In this study, centromere numerical abnormality (CNA) was investigated by fluorescence in situ hybridization (FISH) in a case of multiple gastric cancer having intratumor histological heterogeneity. The different profiles as determined using a total of 20 specific probes on 4 multifocal lesions in the stomach confirmed the multi‐clonality of these tumors. FISH with probes specific for chromosomes 10, 11, 16 and 18 revealed intratumor heterogeneity of the CNA, which corresponded to the histological heterogeneity. Our report clearly demonstrates, for the first time, intratumor heterogeneity of CNA and its association with the histological picture, and substantiates the applicability of the MW‐assisted FISH protocol to paraffin‐embedded pathological specimens.