Open AccessThe Specific Expression of Three Novel Splice Variant Forms of Human Metalloprotease‐like Disintegrin‐like Cysteine‐rich Protein 2 Gene in Brain Tissues and Glioma
Author(s) -
Harada Taishi,
Nishie Akihiro,
Torigoe Kiyoyuki,
Ikezaki Kiyonobu,
Shono Tadahisa,
Maehara Yoshihiko,
Kuwano Michihiko,
Wada Morimasa
Publication year - 2000
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.2000.tb00877.x
Subject(s) - biology , exon , gene , complementary dna , microbiology and biotechnology , alternative splicing , cdna library , gene expression , genetics
We have previously identified 67 exons on a yeast artificial chromosome contig spanning 1.5 Mb around the multidrug resistance 1 gene region of human chromosome 7q21.1. In this study, we identified three novel cytoplasmic variants (MDC2‐γ, MDC2‐δ, and MDC2‐γ) of the human metalloprotease‐like disintegrin‐like cysteine‐rich protein 2 (MDC2) among these exons by screening a human brain cDNA library and also by using a reverse transcription polymerase chain reaction. Genomic sequence analysis strongly supported the idea that the variations in the cytoplasmic domain were generated by alternative splicing. The expression of MDC2 variant forms in human brain tissue and gliomas was examined by reverse transcription polymerase chain reaction and RNase protection assay. MDC2‐& ccar was expressed only in the cortical and hippocampal regions in human brain, but not in gliomas. In contrast, MDC2‐γ was a major form expressed in human gliomas. Specific expression of these cytoplasmic variants of MDC2 in human brain and its malignancies is discussed.