Open AccessHigh Rate of Induction of Human Autologous Cytotoxic T Lymphocytes against Renal Carcinoma Cells Cultured with an Interleukin Cocktail
Author(s) -
Liu Shu Qin,
Kawai Koji,
Shiraiwa Hiroshi,
Hayashi Hitoshi,
Akaza Hideyuki,
Hashizaki Kazuko,
Shiba Reiko,
Saijo Kaoru,
Ohno Tadao
Publication year - 1998
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1998.tb00515.x
Subject(s) - ctl* , cytotoxic t cell , immunotherapy , lymphokine activated killer cell , interleukin 2 , renal cell carcinoma , immunology , peripheral blood mononuclear cell , carcinoma , major histocompatibility complex , cancer research , medicine , cd8 , biology , in vitro , antigen , cytokine , pathology , interleukin 21 , immune system , biochemistry
A high rate of induction (9 of 10 cases) of human autologous cytotoxic T lymphocytes (CTL) was achieved in vitro from peripheral blood mononuclear cells of renal carcinoma patients by applying an interleukin (IL)‐cocktail consisting of IL‐1, ‐2, ‐4, and ‐6. The CTL specifically lysed their own target carcinoma cells within 24 h but did not kill neighboring autologous normal kidney cells or allogeneic renal cancer cell lines. In the case of TUHR4TKB, for which autologous CTL were not induced, no expression of MHC class‐I molecules was observed on the surface of these carcinoma cells, although they were sensitive to autologous natural killer cells. The results imply that adoptive immunotherapy for metastasized renal carcinoma will be feasible with autologous CTL in combination with natural killer cells.