Open AccessHeterogeneity of DNA Ploidy Pattern in Carcinoma of the Gallbladder: Primary and Metastatic Sites
Author(s) -
Futakawa Noriaki,
Kimura Wataru,
Ando Hidehiko,
Muto Tetsuichiro,
Esaki Yoshiyuki
Publication year - 1997
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1997.tb00465.x
Subject(s) - gallbladder , carcinoma , pathology , epithelioma , biology , metastatic carcinoma , ploidy , metastasis , medicine , cancer research , cancer , genetics , gene
There are few detailed reports on the heterogeneity of the nuclear DNA ploidy pattern in carcinoma of the gallbladder. We studied twelve autopsied cases who died of extended gallbladder carcinoma. Multiple samples were taken from the primary site (Pri), from direct invasion of the liver ( Hin f), from hematogenous metastasis to the liver (H), from lymphatic metastasis (LN) and from peritoneal dissemination (P). The DNA ploidy pattern was investigated by image cytometry. Heterogeneity of the DNA ploidy pattern in Pri, Hin f, H, LN and P was found in 7/11, 2/10, 5/10, 2/6 and 3/6 cases, respectively. Aneuploidy was more frequently found in Hin f than at the Pri. The DNA index of Hin f was significantly higher than that of Pri. Several stcmlincs, with different quantities of DNA, were found in Pri. Most of these stemlines were also observed in other sites. These facts may suggest that polyclonal cancer cells rather than one cancer cell or monoclonal cancer cells of a Pri metastasize or infiltrate, and that various polyclonal cancer cells proliferate to different degrees under different circumstances.