Open AccessTwo Distinct Human Uterine Cervical Epithelial Cell Lines Established after Transfection with Human Papillomavirus 16 DNA
Author(s) -
Ohta Yujiro,
Tsutsumi Kouichiro,
Kikuchi Keiji,
Yasumoto Shigeru
Publication year - 1997
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1997.tb00432.x
Subject(s) - transfection , biology , cell culture , carcinogenesis , population , cervix , telomerase , in situ hybridization , virology , polymerase chain reaction , microbiology and biotechnology , cell , epithelium , human papillomavirus , telomerase reverse transcriptase , cancer research , gene , genetics , medicine , gene expression , cancer , environmental health
We have established two distinct human cervical cell lines, NCC16 and NCE16, after traiisfecting human papillomavirus type 16 (HPV16) DNA into normal human ecto‐cervical and endo‐cervical epithelial cells, respectively. Both lines expressed HPV16 E6 and E7 as detected hy reverse transcriptase‐polymerase chain reaction and northern Wot hybridization. These cells have been passaged for over 100 population doublings and express strong telomerase activity. Neither cell line was tumorigenic in athymic nu/nu mice. However, both NCC16 and NCE16 developed abnormally stratified architectures following implantation with a silicon membrane sheet in the back of athymic nude mice. The former cells were pathohistologically similar to carcinoma, while the latter produced Alcian‐blue positive cells, suggesting the occurrence of metaplastic changes. These distinct cell lines offer a useful model system for the study of cervical carcinogenesis and of its regulatory mechanism after HPV infection in different regions of the uterine cervix.