Open AccessTransforming Growth Factor‐β and Hepatocyte Growth Factor Produced by Gastric Fibroblasts Stimulate the Invasiveness of Scirrhous Gastric Cancer Cells
Author(s) -
Inoue Tohru,
Chung YongSuk,
Yashiro Masakazu,
Nishimura Shigehiko,
Hasuma Tadayoshi,
Otani Shuzo,
Sowa Michio
Publication year - 1997
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1997.tb00360.x
Subject(s) - hepatocyte growth factor , paracrine signalling , biology , autocrine signalling , cancer research , transforming growth factor , fibroblast , cancer cell , growth factor , cell culture , cancer , pathology , endocrinology , medicine , receptor , biochemistry , genetics
Scirrhous gastric carcinoma is characterized by cancer cells that infiltrate rapidly in the stroma with extensive growth of fibroblasts. In the present study, we examined the effect of gastric fibroblasts on the invasiveness of a Scirrhous gastric cancer cell line, OCUM‐2D, using an invasion assay. Gastric fibroblast‐derived conditioned medium (CM) significantly stimulated the invasiveness of OCUM‐2D cells, as did transforming growth factor‐ β (TGF‐ β ) and hepatocyte growth factor (HGF). The stimulating activity of gastric fibroblast‐derived CM was inhibited significantly by anti‐TGF‐ β neutralizing antibody or anti‐HGF neutralizing antibody. TGF‐ β and HGF were detected in the gastric fibroblast‐derived CM, and TGF‐ β receptor and C‐met (HGF receptor) were expressed on OCUM‐2D cells. Thus, TGF‐ β and HGF produced by gastric fibroblasts appear to affect the invasiveness of scirrhous gastric cancer cells. TGF‐ β was also detected in the conditioned medium derived from OCUM‐2D cells, though HGF was not. TGF‐ β appears to affect the invasiveness of OCUM‐2D cells in both paracrine and autocrine fashions.