Open AccessInduction of Aberrant Crypt Foci and Flat‐type Adenocarcinoma in the Colons of Dogs by N‐Ethyl‐N'‐nitro‐N‐nitrosoguanidine and Their Sequential Changes
Author(s) -
Sugiyama Kenji,
Oda Yasushi,
Otori Kazuhiko,
Kato Shigeharu,
Hasebe Takahiro,
Fujii Takahiro,
Tajiri Hisao,
Esumi Hiroyasu
Publication year - 1997
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1997.tb00311.x
Subject(s) - beagle , adenocarcinoma , aberrant crypt foci , methylnitronitrosoguanidine , pathology , lesion , crypt , medicine , biopsy , gastroenterology , carcinogenesis , azoxymethane , carcinogen , biology , colorectal cancer , colonic disease , cancer , biochemistry , genetics , mutant , gene
Sequential endoscopic observation of dog colons was performed during colon carcinogenesis. Two beagle dogs were given suppositories containing N‐ethyl‐N'‐nitro‐N‐nitrosoguanidine (ENNG) every day for five months. In month 3, aberrant crypt foci (ACF), a putative preneoplastic lesion, were found in the colons of both dogs, but not in an untreated dog. The frequency of ACF increased until month 10, and then decreased. In month 9, very small lesions, less than 1 mm in diameter, which were similar to human early flat tumors, were first noticed. One of these lesions grew to about 7 mm in size without a change in its shape for 10 months. There were more than ten flat‐type tumors in the two dogs, but such lesions were not found in the untreated dog. By biopsy, two of the lesions were proved to be well‐differentiated adenocarcinomas histologically. Four polypoid lesions were found in one of the carcinogen‐treated dogs. Thus, flat‐type adenocarcinomas were induced in the dog colon by ENNG, and their development was followed by magnifying endoscopy.