Open AccessExpression of p21 waf‐1/cip‐1 Is Significantly Induced in the Livers of LEC Rats with Chronic Liver Injury
Author(s) -
Sawada Norimasa,
Kojima Takashi,
Obata Hiroyuki,
Isomura Hiroshi,
Atsumi Shinichi,
Sawaki Masakuni,
Tsuzuki Naoto,
Tobioka Hirotoshi,
Kokai Yasuo,
Satoh Masaaki,
Mori Michio
Publication year - 1996
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1996.tb03117.x
Subject(s) - western blot , hepatocellular carcinoma , liver injury , biology , microbiology and biotechnology , immunohistochemistry , northern blot , cell growth , pathology , gene expression , cancer research , endocrinology , medicine , gene , immunology , biochemistry
It is reported that hepatocytes isolated from LEC rats with chronic liver injury show reduced growth activity in primary culture. To elucidate the molecular basis of this phenomenon, we examined expression of p21 waf‐1/cip‐1 and p27, cyclin‐dependent kinase inhibitors, by northern blot analysis. The expression of p21 waf‐1/cip‐1 in the LEC rat liver was 3‐fold higher than that of age‐matched SD rat liver, while there was no significant difference in p27 expression level. Western blot analysis also revealed a significant increase in p2l waf‐1/cip‐1 in the nuclear matrix fraction of the LEC rat liver. Immunohisto‐chemically, p21 waf‐1/cip‐1 was detected in the nuclei of normal LEC rat hepatocytes, but not in those of hepatocellular carcinoma cells, suggesting selective growth of neoplastic hepatocytes.