Open AccessEfficient Method for Rapid Induction of Aberrant Crypt Foci in Rats with 2‐Amino‐1‐methyl‐6‐phenylimidazo[4,5‐ b ]pyridine
Author(s) -
Ochiai Masako,
Nakagama Hitoshi,
Watanabe Masatoshi,
Ishiguro Yukiko,
Sugimura Takashi,
Nagao Minako
Publication year - 1996
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1996.tb03105.x
Subject(s) - crypt , aberrant crypt foci , chemistry , pyridine , pathology , microbiology and biotechnology , biology , biochemistry , medicine , stereochemistry , medicinal chemistry , cancer , colonic disease , colorectal cancer
2‐Amino‐1‐methyl‐6‐phenylimidazo[4,5‐ b ]pyridine (PhIP) induces aberrant crypt foci (ACFs) as well as colon cancer in F344 male rats. Conditions allowing rapid development of ACFs over a short period were investigated. F344 male rats were fed 400 ppm of PhIP‐HCl in a low‐fat diet (LF) for 2 weeks and then given a PhIP‐free, high‐fat diet containing PRIMEX (HF‐PR) or safflower oil, or PhIP‐free LF for 4 or 12 weeks. Rats fed HF‐PR for 4 weeks gave the highest number of ACFs/rat (3.3) and their size in terms of aberrant crypts/ACF(2.7) was much larger than that obtained with conventional continuous feeding of PhIP for 25 weeks in the CE‐2 diet. Therefore, 2 weeks of dietary exposure to 400 ppm of PhIP‐HCl, followed by HF‐PR for 4 weeks, is a practical and convenient method for obtaining ACFs. This protocol should be useful for studies of the early phase of colon carcinogenesis.