Open AccessCharacterization of Transendothelial Migratory Lymphokine‐activated Killer Cells
Author(s) -
Nakano Koji,
Eura Masao,
Chikamatsu Kazuaki,
Masuyama Keisuke,
Ishikawa Takeru
Publication year - 1996
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1996.tb00235.x
Subject(s) - lymphokine activated killer cell , lymphokine , cytotoxic t cell , umbilical vein , cytotoxicity , immunology , chemistry , microbiology and biotechnology , biology , in vitro , immune system , interleukin 21 , biochemistry
We examined the killing activity of transmigrated lymphokine‐activated killer (LAK) cells and their surface molecules associated with both transendothelial migration and cytotoxicity, using human umbilical vein‐derived endothelial cell (HUVEC) monolayers on fibronectin with gelatin separating the upper chamber from the lower chamber. Migratory LAK cells were significantly more cytotoxic to Daudi target cells, expressed more LFA‐1, and were more likely to be positive for CD2, compared to those LAK cells not adherent to the HUVEC monolayer. In contrast, in the absence of the HUVEC monolayer, there was no difference in LAK activity between migratory and non‐adherent LAK cells. These results indicate that the interaction between LAK cells and the HUVEC monolayer allows selective migration of LAK cells with cytotoxic activity that is enhanced with respect to some surface molecules.