Open AccessNon‐radioisotopic and Semi‐quantitative Procedure for Terminal Repeat Amplification Protocol
Author(s) -
Ohyashiki Junko H.,
Ohyashiki Kazuma,
Sano Tetsuro,
Toyama Keisuke
Publication year - 1996
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1996.tb00225.x
Subject(s) - terminal (telecommunication) , protocol (science) , microbiology and biotechnology , chemistry , computational biology , biology , computer science , medicine , pathology , telecommunications , alternative medicine
We have used fluorescence‐labeled primers and an auto‐sequencer to detect telomerase activity quickly and easily. The current procedure is superior to the original telomeric repeat amplification protocol in several respects: 1) the result is obtained in real time during electrophoresis, 2) semi‐quantitative results are possible without using a photo‐capture system, and 3) no radioisotope is needed.