Open AccessAbsence of a Mutation of the p21/WAF1 Gene in Human Lung and Pancreatic Cancers
Author(s) -
Shimizu Tatsunori,
Miwa Wataru,
Nakamori Shoji,
Ishikawa Osamu,
Konishi Yoichi,
Sekiya Takao
Publication year - 1996
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1996.tb00217.x
Subject(s) - mutation , lung , cancer research , human lung , medicine , gene , pancreatic disease , lung cancer , pathology , biology , oncology , pancreas , genetics
The expression of a negative regulator of the cell cycle, p21 WAF1 protein, is trans‐activated hy wild‐type p53, but not by the mutant protein. Therefore, mutations of the p 53 and WAF1 genes may be complementary. We examined DNAs from 70 human primary lung (63 of NSCLC and 7 of SCLC) and 24 pancreatic cancers (19 primary cancers and 5 cell lines) for mutations of the WAF1 gene. No mutations were detected in any samples examined, regardless of the mutational state of the p 53 gene. The results suggested that aberrations of the coding sequence of the WAF1 gene are not associated with carcinogenesis in lung and pancreatic cancers.