Open AccessPresence of Streptococcus DNA Sequence in Surgical Specimens of Gastric Cancer
Author(s) -
Sasaki Hiroki,
Igaki Hiroyasu,
Ishizuka Tomoki,
Kogoma Yukiko,
Sugimura Takashi,
Terada Masaaki
Publication year - 1995
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1995.tb03086.x
Subject(s) - southern blot , biology , 16s ribosomal rna , mycoplasma , ribosomal dna , microbiology and biotechnology , sequence analysis , dna , gene , genetics , phylogenetic tree
In Southern blot analysis using Mycoplasma 16S ribosomal DNA (rDNA) as a probe, positive signals were detected in DNA samples from surgical specimens of gastric cancers. The DNA that hybridized to Mycoplasma 16S rDNA was eluted from the gel, cloned and sequenced. The cloned sequence was identical to 16S rDNA of Streptococcus anginosus . In Southern blot analysis with the S. anginosus 16S rDNA fragment as a new probe, positive signals were detected in 9 (20%) out of 43 cases of gastric cancer.