Open AccessFrequent Genetic Instability in Small Intestinal Carcinomas
Author(s) -
Hibi Kenji,
Kondo Ken,
Akiyama Seiji,
Ito Katsuki,
Takagi Hiroshi
Publication year - 1995
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1995.tb03064.x
Subject(s) - microsatellite instability , carcinoma , polymerase chain reaction , pathogenesis , pathology , biology , colorectal cancer , cancer , cancer research , microsatellite , medicine , gene , genetics , allele
To determine whether genetic instability plays a part in the development of digestive tract carcinomas, we analyzed 3 microsatellite loci isolated from tumors and surrounding normal tissue samples obtained during surgery. The polymerase chain reaction (PCR) technique was used to assess differences between tumor and matched normal DNAs. Replication errors (RERs) were observed in 3 of the 29 cases (10%) of gastric carcinoma and in 11 of the 72 cases (15%) of colorectal carcinoma. None of the 13 (0%) esophageal carcinoma cases showed any RER, but 5 of the 11 cases of small intestinal carcinoma (45%) had RERs, a significantly frequent finding. These results suggest that genetic instability plays an important role in the pathogenesis of small intestinal carcinomas.