Open AccessFrequent Loss of the Cyclin‐dependent Kinase‐4 Inhibitor Gene in Human Gliomas
Author(s) -
Gomi Akira,
Sakai Ryuichi,
Ogawa Seishi,
Shinoda Souji,
Hirai Hisamaru,
Masuzawa Toshio
Publication year - 1995
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1995.tb03062.x
Subject(s) - cancer research , kinase , cyclin dependent kinase , gene , cyclin dependent kinase 4 , medicine , biology , protein kinase a , genetics , cell cycle , cyclin dependent kinase 2
Studies have shown that homozygous deletion of the cyclin‐dependent kinase‐4 inhibitor (CDK41) gene, which is mapped to chromosome 9p21, is frequently observed in various types of human cancers. Here we report that the CDK4I gene was deleted in gliomas. Eight cell lines derived from glioblastomas and samples from 14 patients with various grades of gliomas were examined by Southern blot analysis. We found that the CDK4I gene was deleted in 7 of 8 (87.5%) cell lines and 7 of 9 (78%) samples from high‐grade glioma patients, whereas it was deleted in 1 of 5 (20%) low‐grade glioma samples. These results suggested that inactivation of the CDK4I gene may play an important role in the progression of human glioma.