Open AccessTyrosine Phosphorylation of the Proto‐oncogene Product Vav and Its Association with the Adapter Grb2/Ash in a Human Leukemia Cell Line UT‐7
Author(s) -
Hanazono Yutaka,
Sasaki Ko,
Odai Hideharu,
Mimura Toshihide,
Mitani Kinuko,
Yazaki Yoshio,
Hirai Hisamaru
Publication year - 1995
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1995.tb03061.x
Subject(s) - guanine nucleotide exchange factor , tyrosine phosphorylation , grb2 , phosphorylation , tyrosine , cancer research , tyrosine kinase , biology , leukemia , signal transducing adaptor protein , signal transduction , microbiology and biotechnology , immunology , biochemistry
The vav proto‐oncogene product (Vav) is expressed exclusively in hematopoietic cells and is reported to have guanine nucleotide exchange activity. Here we report that granulocyte‐macrophage colonystimulating factor, interleukin‐3, and erythropoietin induce tyrosine phosphorylation of Vav in a human leukemia cell line UT‐7. Tyrosine phosphorylation of Vav is rapid and transient; it occurs within 1 min of the stimulation and at physiological concentrations of the factors. Furthermore, we show that Vav is constitutively associated with the adapter molecule Grb2/Ash in UT‐7. These data suggest that tryosine kinases, the adapter Grb2/Ash, and the guanine nucleotide exchange factor Vav are members of a signaling pathway leading to Ras activation in hematopoietic cells.