Open AccessMannose‐binding Protein Recognizes Glioma Cells: In vitro Analysis of Complement Activation on Glioma Cells via the Lectin Pathway
Author(s) -
Fujita Takashi,
Taira Satoshi,
Kodama Namio,
Matsushita Misao,
Fujita Teizo
Publication year - 1995
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1995.tb03038.x
Subject(s) - complement system , glioma , mannan binding lectin , lectin pathway , lectin , in vitro , biology , mannose , ficolin , cell culture , classical complement pathway , serine protease , flow cytometry , microbiology and biotechnology , biochemistry , cancer research , protease , immunology , antibody , enzyme , genetics
The lectin pathway is a novel pathway for activation of the complement cascade, which is initiated by the binding of mannose‐binding protein (MBP) to its carbohydrate ligands. We investigated whether the complement system was activated in vitro by glioma cells through this pathway to the C3 level. MBP was found to bind to all six glioma cell lines tested by using flow cytometric analysis. Binding of a complex of MBP‐associated serine protease and MBP was observed in two of the cell lines examined, thereby resulting in C4 consumption. Activation of C3 was hemolytically evaluated in these two lines. C3 consumption was also observed in one. Based on these results, it is likely that recognition by MBP followed by complement activation occurs in certain glioma cell lines.