Open AccessInhibition of 1,2‐Dimethylhydrazine‐induced Oxidative DNA Damage by Green Tea Extract in Rat
Author(s) -
Inagake Masao,
Yamane Tetsuro,
Kitao Yoshitaka,
Oya Kazuhiko,
Matsuraoto Hirohiko,
Kikuoka Norikazu,
Nakatani Hirohisa,
Takahashi Toshio,
Nishimura Hiroshi,
Iwashiraa Akio
Publication year - 1995
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1995.tb03027.x
Subject(s) - 1,2 dimethylhydrazine , carcinogen , dimethylhydrazine , dna damage , chemistry , green tea extract , azoxymethane , subcutaneous injection , kidney , oxidative damage , oxidative phosphorylation , oxidative stress , dna , green tea , biochemistry , medicine , endocrinology , food science , biology , carcinogenesis , colorectal cancer , cancer , gene
Following subcutaneous injection of 1,2‐dimethylhydrazine (DMH), which is carcinogenic to rat colon and liver, to Sprague‐Dawley rats, a significant increase of 8‐hydroxydeoxyguanosine (8‐OHdG) was observed in the DNA of colonic mucosa and liver. The 8‐OHdG formation reached the maximal level at about 24 h after the DMH injection. On the other hand, no increase of 8‐OHdG was observed in the DNA of the kidney. Drinking green tea extract (GTE) for ten days prior to the DMH injection significantly inhibited the formation of 8‐OHdG in the colon. These findings demonstrate that DMH causes oxidative damage to the DNA of its target organ, and that GTE protects colonic mucosa from this oxidative damage.