Open AccessT‐Cell Receptor Gene Structures of HLA‐A26‐restricted Cytotoxic T Lymphocyte Lines against Human Autologous Pancreatic Adenocarcinoma
Author(s) -
Ueda Daisuke,
Sato Noriyuki,
Matsuura Akihiro,
Sasaki Aya,
Takahashi Shuji,
Ikeda Hideyuki,
Wada Yoshimasa,
Kikuchi Kokichi
Publication year - 1995
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1995.tb02454.x
Subject(s) - t cell receptor , ctl* , cytotoxic t cell , biology , t lymphocyte , microbiology and biotechnology , adenocarcinoma , beta (programming language) , gene rearrangement , t cell , lymphocyte , immunology , antigen , cancer research , gene , immune system , genetics , cancer , in vitro , computer science , programming language
We isolated two cytotoxic T lymphocyte (CTL) lines, which were independently obtained by mixed lymphocyte‐tumor cell culture from tumor‐infiltrating lymphocytes of a patient with pancreatic adenocarcinoma. Both lines behaved identically in all the functional aspects tested and appeared to be HLA‐A26‐restricted. We analyzed their T cell receptor (TCR) gene structures, including V‐(D)‐J junctional sequences, which are unique to each T‐cell clonotype and contribute to TCR diversity. Each line consisted of a clonal T‐cell expressing Vα18 and Vβ7. The α chain gene was composed of Vα18/ JαF/Cα and the, β‐chain gene, of Vβ7.1/Dβ/Jβ1.4/Cβ2. The sequences were all in‐frame and therefore should yield functional transcripts. The junctional sequences were identical between the two lines. These data suggested that the two CTL clones having the same CDR3 had descended from a common precursor lymphocyte. The clonal expansion of CTL lines with the identical CDR3 implies that they are directed against the same tumor antigen, which seemed to be immunologically dominant in the specific interaction between the CTL and the autologous pancreatic adenocarcinoma.