Open AccessFrequent Amplification of the Cyclin E Gene in Human Gastric Carcinomas
Author(s) -
Akama Yoshihiko,
Yasui Wataru,
Yokozaki Hiroshi,
Kuniyasu Hiroki,
Kitahara Kenji,
Ishikawa Takenori,
Tahara Eiichi
Publication year - 1995
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1995.tb02442.x
Subject(s) - cyclin d1 , cyclin d , biology , cancer research , cyclin b , cyclin , gene duplication , microbiology and biotechnology , cyclin a2 , cancer , gene , cell cycle , genetics
We searched for genetic alterations of the cyclin D1 and cyclin E genes in 45 human gastric carcinoma tissues. Expression of cyclin E mRNA and protein was also analyzed in eight of them by Northern and Western blots and immunohistochemical staining. The cyclin E gene was amplified 3–10 fold in seven gastric cancer tissues (15.6%), of which six were advanced gastric cancers. All of the cases with the cyclin E gene amplification displayed lymph node metastasis. Moreover, the case with the gene amplification overexpressed the cyclin E mRNA and protein. One of eight gastric cancer cell lines, MKN‐7, shared the cyclin E gene amplification, and all of the gastric cancer cell lines expressed high levels of the cyclin E mRNA and protein even without gene amplification. Amplification of the cyclin D1 gene was not observed in any of the gastric carcinoma tissues or gastric carcinoma cell lines. These results suggest that the gene amplification and overexpression of cyclin E play an important role in the abnormal growth and progression of gastric carcinoma.