Open AccessMonoclonal Antibody against PRADl/Cyclin Dl Stains Nuclei of Tumor Cells with Translocation or Amplification at BCL‐1 Locus
Author(s) -
Banno Shogo,
Yoshikawa Kazuhiro,
Nakamura Shigeo,
Yamamoto Kazuhito,
Seito Tsutomu,
Nitta Masakazu,
Takahashi Toshitada,
Ueda Ryuzo,
Seto Masao
Publication year - 1994
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1994.tb02969.x
Subject(s) - biology , microbiology and biotechnology , cyclin d1 , monoclonal antibody , immunofluorescence , chromosomal translocation , staining , antibody , antigen , cell nucleus , gene product , cytoplasm , cell , gene , cell cycle , gene expression , biochemistry , immunology , genetics
Mouse monoclonal antibodies were produced against the bacterial product encoded by human PRAD1/ cyclin Dl gene, which is known to be involved in tumors with translocation or amplification at BCL‐1 locus of 11ql3. The immunizing antigens used were GST‐PRAD1 and T7 gene 10‐PRAD1 fusion products. Four antibodies were reactive with both PRAD1 fusion products and cell lysates of B‐cell tumor cell lines with t(ll;14)(ql3;q32) and a breast cancer cell line with 11ql3 amplification, on immunoblotting. An immunofluorescence study showed that only one of them stained nuclei of cells with 11q13 abnormalities. Since this antibody proved applicable for conventional paraffin‐embedded tissue sections, immunohistologic staining of various lymphoma tissues was performed. Eight of 11 mantle cell lymphomas showed intermediate to strong positivity and 6 of the positive cases demonstrated characteristic staining patterns that were either predominantly nuclear or both nuclear and cytoplasmic. The nuclear staining pattern was not observed with other types of lymphoma and thus may correlate with PRAD1 mRNA overexpression.