Open AccessVariant Translocation of the BCL6 Gene to Immunoglobulin k Light Chain Gene in B‐Cell Lymphoma
Author(s) -
Suzuki Ken,
Miki Tohru,
Kawamata Norihiko,
Hirosawa Shinsaku,
Yoshizawa Kaname,
Kiyosawa Kendo,
Aoki Nobuo
Publication year - 1994
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1994.tb02968.x
Subject(s) - chromosomal translocation , biology , breakpoint , microbiology and biotechnology , genetics , bcl6 , derivative chromosome , gene duplication , immunoglobulin heavy chain , immunoglobulin gene , chromosome , gene , gene rearrangement , chromosome 22 , antibody , b cell , germinal center
A lymphoma cell line with a variant type of translocation, t(2;3)(pll;q27), was established from a patient who had received liver transplantation. To elucidate the molecular mechanism of the t(2;3)(pll;q27) chromosomal translocation, we compared the structures of both derivative (der) chromosomal breakpoints with those of their germline predecessors. We noted that the BCL6 gene on chromosome 3 was juxtaposed with the immunoglobulin k light chain (Ig k ) gene on chromosome 2 in a head‐to‐head configuration. The breakpoint of the BCL6 gene was within a previously reported breakpoint cluster region. The breakpoint on chromosome 2 was within the intron between the leader (L) and variable (V) sequences of one of the V k genes, which was fused to the J k 3 (J=joining) segment. At chromosomal junctures, a direct repeat duplication of chromosome 3 sequences and a deletion of chromosome 2 sequences were discovered. These results are consistent with a translocation model with illegitimate pairing of staggered double‐stranded DNA breaks at 3q27 and 2pll, repair, and ligation to generate der(3) and der(2) chromosomes.