Open AccessImmunohistochemical Analysis of Cyclin D1 Protein in Hematopoietic Neoplasms with Special Reference to Mantle Cell Lymphoma
Author(s) -
Nakamura Shigeo,
Seto Masao,
Banno Shogo,
Suzuki Susumu,
Koshikawa Takashi,
Kitoh Kuniyoshi,
Kagami Yoshitoyo,
Ogura Michinori,
Yatabe Yasushi,
Kojima Masaru,
Motoori Tadashi,
Takahashi Toshitada,
Ueda Ryuzo,
Suchi Taizan
Publication year - 1994
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1994.tb02940.x
Subject(s) - cyclin d1 , mantle cell lymphoma , mantle zone , biology , lymphoma , immunohistochemistry , pathology , staining , cyclin , cytoplasm , antibody , cell , b cell , cell cycle , immunology , medicine , microbiology and biotechnology , germinal center , genetics
Immunohistochemical expression of PRAD1/cyclin D1 protein has been investigated in 106 tissue specimens of 104 cases of lymphoma, non‐neoplastic lymphoid disorders and other hematologic malignancies by employing the monoclonal antibody 5D4 with formalin‐fixed paraffin‐embedded sections, using the microwave oven heating method. Positive neoplastic cells were found in 60 (74%) of 81 cases of non‐Hodgkin's lymphoma. The positivity pattern was nuclear in 17 (85%) of 20 cases of mantle cell lymphoma in which cytoplasmic staining was also seen. This pattern of cyclin D1 positivity was in contrast to the negative staining of normal reactive mantle zones. In the other cases, positivity appeared to lie within the cell cytoplasm without nuclear staining, and most of the nodal follicular and diffuse B‐cell lymphomas variously expressed PRAD1/cyclin D1. In contrast, the reaction was absent in a significant number of T‐cell and extranodal B‐cell lymphomas. Immunolocalization of PRAD1/cyclin D1 expression appears to be a useful diagnostic adjunct to discriminate mantle cell lymphoma from other non‐Hodgkin's lymphomas.