Open AccessDifference in Allelic Expression of Genes Probably Associated with Tumor Progression in Murine Fibrosarcomas and Cell Lines
Author(s) -
Ohtsuka Hiroshi,
Mafune Yoshiro,
Tsunashima Katsumasa,
Takahashi Hideaki,
Kominami Ryo
Publication year - 1994
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1994.tb02899.x
Subject(s) - allele , biology , loss of heterozygosity , gene , tumor progression , microbiology and biotechnology , gene expression , phenotype , genetics , cell culture , cancer research
Allelic expression was examined by single‐strand conformation polymorphism analysis in murinc fibrosarcomas from inter‐subspecific F 1 mice between C57BL/6 and MSM. Ten genes encoding p53, mdni2, E‐cadherin, 72 kD metalloproteinase and its inhibitor (Timp2), thymidine kinasc and four glucose transporters (Gluts) were examined. These genes were chosen because of their probable association with tumor development and progression. In some of the tumors and cell lines, p53, E‐cadherin and Glut3 genes showed remarkable differences in allelic expression, one allele being poorly expressed. The allele‐specificity persisted in nine cell lines obtained by repeated transplantations from one tumor. These results suggest that expression of some genes is allele‐specific in tumor cells and the pattern of specificity is stable. Such a decrease or a loss of expression in one of the alleles may be functionally equivalent to the loss of heterozygosity of the gene, and therefore this may confer malignant properties on tumor cells. It is also suggested that differential expression of two alleles is a common event in tumor cells.