Open AccessDetection and Cloning of Human Papillomavirus DNA Associated with Recurrent Respiratory Papillomatosis in Thailand
Author(s) -
Ushikai Masato,
Fujiyoshi Toshinobu,
Kono Motoko,
Antrasena Soontorn,
Oda Hiroshi,
Yoshida Hiroki,
Fukuda Katsunori,
Furuta Shigeru,
Hakura Akira,
Sonoda Shunro
Publication year - 1994
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1994.tb02417.x
Subject(s) - recurrent respiratory papillomatosis , papillomatosis , polymerase chain reaction , clone (java method) , southern blot , virology , dna , biology , recombinant dna , molecular cloning , papillomaviridae , genomic dna , microbiology and biotechnology , papilloma , medicine , gene , pathology , genetics , cancer , cervical cancer , complementary dna
Recurrent respiratory papillomatosis (RRP) is highly prevalent in Thailand. In this study, we examined the presence of human papillomavirus (HPV) DNA in 25 RRP patients in Thailand by means of dot blot analysis and/or polymerase chain reaction. Eighty‐four percent (21/25) of cases and 4% (1/25) of cases were positive for HPV‐11 DNA and HPV‐6 DNA, respectively. Three cases (3/25) were negative for all of the examined HPV types. No cases were positive for HPV‐16 or 18. Furthermore, we isolated the recombinant HPV‐11 DNA clone from a genomic library constructed with the DNA of RRP tissue. The restriction map of the cloned HPV DNA was identical with the map of known HPV‐11 DNA. These results suggest at least that no specific HPV type or subtype is likely to be associated with RRP in Thailand.