Open AccessRapid Polymerase Chain Reaction Assay to Detect Variation in the Extent of Gene‐specific Damage between Cisplatin‐ or VP‐16‐resistant and Sensitive Lung Cancer Cell Lines
Author(s) -
Oshita Fumihiro,
Saijo Nagahiro
Publication year - 1994
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1994.tb02412.x
Subject(s) - polymerase chain reaction , cisplatin , lung cancer , gene , biology , microbiology and biotechnology , cancer research , cell culture , genetics , pathology , medicine , chemotherapy
We previously established a rapid and facile polymerase chain reaction (PCR)‐stop assay for quantitation of specific gene damage in very small numbers of cells. The present study investigated whether the PCR‐stop assay was able to detect variation in the extent of DNA damage in transcribed active genes between cisplatin‐ or VP‐16‐resistant and sensitive cells. The assay demonstrated that about twice as much genetic damage occurs in PC‐9 cells than in cisplatin‐resistant PC‐9/CDDP cells following cisplatin exposure and about 4.6 times more damage occurs in H69 than in VP‐16‐resistant H69/VP cells following VP‐16 exposure. These results show that DNA damage, as detected by PCR‐stop assay, correlates with cytotoxicity. In conclusion, the PCR‐stop assay could be useful in detecting variation in DNA damage in specific genes.