Open AccessAnalysis of T Cell Receptor Variability in Fresh Tumor‐infiltrating Lymphocytes from Human Head and Neck Cancer
Author(s) -
Chikamatsu Kazuaki,
Eura Masao,
Nakano Koji,
Kanzaki Yuichi,
Matsuoka Hiroaki,
Masuyama Keisuke,
Ishikawa Takeru
Publication year - 1994
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1994.tb02405.x
Subject(s) - t cell receptor , tumor infiltrating lymphocytes , biology , head and neck squamous cell carcinoma , pathology , t cell , cancer , population , cancer research , head and neck cancer , immunology , microbiology and biotechnology , immune system , medicine , cd8 , genetics , environmental health
In this study, we analyzed T cell receptor (TCR) gene rearrangements in tumor‐infiltrating lymphocytes (TIL) freshly obtained from 15 patients with head and neck cancer using the reversely transcribed polymerase chain reaction (RT‐PCR) method. These TILs showed preferential expression of Vα10, Vα8 and Vα1, detected in 13 (87%), 11 (73%), and 9 cases (60%), respectively. The TCRVβ gene revealed diversity without preferential usage. The head and neck region is exposed to bacteria and viruses, so it is possible that the tumor site can become infected and accumulate T cells involved in infection and inflammation. Therefore, we also investigated TCR gene usage in T cells infiltrating in chronic sinusitis mucosa to address the question of whether the Vα1, Vα8, and Vα10 subfamilies are characteristic in TIL from squamous cell carcinoma of head and neck. TCR Vα10 gene usage was also the most common in Vα segment in T cells infiltrating the sinus mucosa, but Vα and Vα8 were not detected in the T cells in sinusitis. These results indicate that the Vα10 subfamily, the preferred T cell population in both TIL and T cells in inflammatory disease, might he involved mainly in inflammation or infection. On the other hand, Vα1 and Vα8 appear to be relatively specific populations for antitumor immunity in head and neck cancer.