Open AccessDetection of Epidermal Growth Factor Receptor Gene Amplification in Human Squamous Cell Carcinomas Using Fluorescence in situ Hybridization
Author(s) -
Shimizu Nobuyoshi,
Wang Yimin,
Minoshima Shinsei,
Ishitoya Junichi
Publication year - 1994
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1994.tb02397.x
Subject(s) - amplicon , biology , fluorescence in situ hybridization , epidermal growth factor receptor , gene duplication , in situ hybridization , epidermal growth factor , microbiology and biotechnology , metaphase , gene , in situ , cancer research , cell culture , pathology , gene expression , cancer , polymerase chain reaction , chromosome , genetics , chemistry , medicine , organic chemistry
The gene for epidermal growth factor receptor (EGFR) is associated with development of certain human cancers. In this study, we employed the improved fluorescence in situ hybridization technique to detect EGFR gene amplification in cell lines and tissue sections from human squamous cell carcinomas. We detected multiple distinct signals as arrayed amplicons on metaphase chromosomes and interphase nuclei of tumor cells. Our results provide a basis for rapid and quantitative DMA diagnosis of the EGFR gene amplification in individual cells of tumor specimens.