Open AccessCorrelation between Tumorigenicity and Expression Levels or Splicing Patterns of Transcripts of the Human Papillomavirus Type 16 E6 Gene
Author(s) -
Inoue Tadao,
Kyo Satoru,
Kiyono Tohru,
Ishibashi Masahide,
Ishiwatari Haruko,
Hwang YongII,
Yutsudo Masuo,
Hakura Akira
Publication year - 1994
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1994.tb02367.x
Subject(s) - rna splicing , messenger rna , open reading frame , biology , gene , alternative splicing , microbiology and biotechnology , transformation (genetics) , gene expression , exon , genetics , rna , peptide sequence
For determination of the correlation between tumorigenicity and the expression levels or splicing patterns of E6 mRNAs of the human papillomavirus type 16 in established cells, a vector containing the intact E6 open reading frame which expresses both unspliced and spliced mRNAs, one expressing only unspliced E6 mRNA, and one expressing both unspliced and spliced mRNAs but producing only truncated E6 proteins were constructed. In transformation assays and analyses of E6 mRNAs, a higher expression level of unspliced E6 mRNA was found to be closely associated with tumorigenicity. Furthermore, it was also related with anchorage‐independent growth and a decreased serum requirement of the cells.