Open AccessInhibitory Effect of Potassium Citrate on Rat Renal Tumors Induced by N‐Ethyl‐N‐hydroxyethylnitrosamine Followed by Potassium Dibasic Phosphate
Author(s) -
Konishi Noboru,
Nishii Kiyoharu,
Hayashi Isao,
Nakaoka Shingo,
Matsumoto Kyoichi,
Yabuno Toru,
Kitahori Yoshiteru,
Hiasa Yoshio
Publication year - 1993
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1993.tb02845.x
Subject(s) - endocrinology , chemistry , medicine , kidney , hypophosphatemia , calcium , nephropathy , biochemistry , diabetes mellitus
Potassium dibasic phosphate (PDP) was administered at a concentration of 10% by weight in basal diet to unilaterally nephrectomized Wistar rats previously given 1000 ppm N‐ethyl‐N‐hydroxyethyl‐nitrosamine (EHEN) in the diet for 2 weeks. To study the effect of alkalinization on renal mineralization, some animals concomitantly received 5% potassium citrate (PC). Feeding PDP alone promoted adenomatous hyperplasias, which were regarded as preneoplastic lesions, as well as renal cell tumors in EHEN‐initiated rats, whereas the addition of PC to PDP diets reduced the promoting effect. Histopathology, serum biochemistry and urinalysis indicated retardation of renal calcium crystallization by PC. Two other phosphate salts, sodium phosphate (SP) and calcium phosphate (CP), were also administered. SP showed a slight promoting effect on adenomatous hyperplasias and a 2‐fold increase in the yield of renal cell tumors, while CP induced a clear reduction of both lesions, over EHEN alone. The promoting effects of both PDP and SP and the inhibitory effect of PC were somewhat correlated to 5‐bromo‐2′‐deoxyuridine labeling indices, the degree of nephropathy, and mineralization in the kidney. Immunohistochemically, the nephropathy induced by phosphate salts was not linked to α zu ‐globulin. A pathogenesis for renal carcinogenesis is suggested in which nephropathy associated with mineralization enhances the development of renal cell tumors.