Open AccessInduction of BCR‐ABL Fusion Genes by in vitro X‐irradiation
Author(s) -
Ito Takashi,
Seyama Toshio,
Mizuno Terumi,
Hayashi Tomonori,
Iwamoto Keisuke S.,
Dohi Kiyohiko,
Nakamura Nori,
Akiyama Mitoshi
Publication year - 1993
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1993.tb02840.x
Subject(s) - fusion gene , breakpoint cluster region , abl , exon , biology , philadelphia chromosome , microbiology and biotechnology , chronic myelogenous leukemia , cancer research , fusion protein , gene , chromosomal translocation , leukemia , genetics , tyrosine kinase , signal transduction , recombinant dna
The Philadelphia chromosome consists of a reciprocal translocation between the ABL oncogene at chromosome 9q34 and the BCR gene at chromosome 22q1l, resulting in the expression of chimeric BCR‐ABL mRNAs specific to chronic myelogenous leukemia (CML). Presence of the fusion gene can be detected with high specificity and sensitivity by means of reverse transcription and polymerase chain reaction. Using this assay, it was possible to detect BCR‐ABL fusion genes induced among HL60 cells after 100 Gy of X‐irradiation in vitro . In total, five fusion gene transcripts were obtained among 10 8 cells examined. These fusion genes contained not only CML‐specific BCR‐ABL rearrangements, but also other forms of BCR‐ABL fusions. These latter genes had junctions of BCR exon 4/ABL exon 2 intervened by a segment of DNA of unknown origin, BCR exon 5/ABL exon 2, and BCR exon 4/ABL exon 2. The results appear to be direct evidence for the induction of the BCR‐ABL fusion gene by X‐irradiation. In terms of leukemogenesis, it appears that only those cells bearing certain CML‐related BCR‐ABL fusion genes are positively selected by virtue of a growth advantage in vivo .