Open AccessA Cancer‐reactive Human Monoclonal Antibody Derived from a Colonic Cancer Patient Treated with Local Immunotherapy
Author(s) -
Yagyu Toshio,
Monden Takushi,
Baba Masashi,
Tamaki Yasuhiro,
Takeda Tsutomu,
Kobayashi Tetsuro,
Shimano Takashi,
Tsuji Yoshiyuki,
Matsushita Hirohisa,
Osawa Hisao,
Murakami Hiroki,
Mori Takesada
Publication year - 1993
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1993.tb02787.x
Subject(s) - monoclonal antibody , cancer , immunostaining , immunohistochemistry , pathology , immunotherapy , antibody , antigen , cancer cell , colonic cancer , lymph node , cancer research , microbiology and biotechnology , colorectal cancer , biology , medicine , immunology
A human monoclonal antibody, YJ‐37 (IgM) was generated through the fusion of human B lymphoblastoid cell line HO‐323 with the regional lymph node lymphocytes from a colonic cancer patient who was treated with a local immunotherapy. This antibody was purified and conjugated with biotin, after which direct immunohistochemical staining was performed. The results revealed that YJ‐37 selectively reacted with colonic cancer (7/19), gastric cancer (3/6), endometrial cancer (1/2) and colonic adenoma (7/13), but not with normal epithelia. Membrane immunofluorescence and FACS analysis also showed that YJ‐37 bound to tumor cell surfaces. Furthermore, the chemical structure of the antigen defined by YJ‐37 was analyzed by means of thin‐layer chromatography immunostaining and ELISA. The results indicated that YJ‐37 reacted with sialylated lacto‐series carbohydrate chains, which have been reported to accumulate in cancer cells.