Open AccessT Cell Response to Embryonal Carcinoma F9 Cells: Induction and Characterization of T Cell Receptor αβ + Double‐negative Cytotoxic T Lymphocytes
Author(s) -
Imada Mie,
Fujimoto Shigeyoshi
Publication year - 1993
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1993.tb02785.x
Subject(s) - cytotoxic t cell , biology , antigen presenting cell , major histocompatibility complex , microbiology and biotechnology , cd8 , interleukin 21 , natural killer t cell , il 2 receptor , lymphokine activated killer cell , antigen , t cell , immune system , immunology , in vitro , biochemistry
We investigated the mechanism of T cell response to marine embryonal carcinoma F9 cells. Thy‐1 + , CD4 − , CD8 − (double‐negative) cytotoxic effector cells were induced in spleen cells obtained from immune A.BY mice to F9 cells, and the cytotoxic activity was major histocompatibility complex (MHC)‐unrestricted. Furthermore, CD4 + T cells were essential for the induction of double‐negative cytotoxic T lymphocytes directed to F9 cells. Most of the double‐negative cytotoxic T lymphocyte lines obtained by long‐term culture of the effector cells had CD3 molecule and T‐cell receptor β chain on their cell surface, and the CDS molecule was found to be involved in target cell recognition. The T cell receptor αβ + double‐negative cytotoxic T lymphocyte line (2A5) also lysed various tumor cells in a non‐MHC‐restricted manner, but did not lyse concanavalin A‐stimulated blasts of 129 strain, from which F9 cells had originated. These results indicate that T cell receptor αβ + double‐negative cytotoxic T lymphocytes induced by F9 cells recognize a common antigen(s) expressed on F9 cells and other tumor cells but not minor histocompatibility antigens.